Summary
Type: SPE
Application #: 304830
Catalog#: 730935 - SPE Cartridges, Chromabond, HR-X, 1ml, PP, 100mg, 30/pk
Detection:
Conditions: Column conditioning: 5 x 1 ml methanol, then 5 x 1 ml bidest. water
(Do not let run the column dry.)
Sample aspiration: The supernantant is passed through the column by a low vacuum
Washing: With 5 x 1 ml bidest. water
Drying: With vacuum water traces are removed (appr. 15 min)
Elution: Analytes are eluted with 3 x 1 ml methanol
Evaporation under nitrogen to dryness at 50 °C; under circumstances freeze at -30 °C
Reconstitution in 100 µl cerebrospinal fluid (CSF) and shaking for 30 sec on Reax
Authors: Gramer, M.
Source: Institut für Pharmakologie, Toxikologie und Pharma